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pcdna myc tcf 4  (Addgene inc)


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    Structured Review

    Addgene inc pcdna myc tcf 4
    Compound 15 inhibits the <t>β‐catenin/TCF‐4</t> interaction. A coimmunoprecipitation assay was performed in HEK293T cells transiently transfected with FLAG‐tagged β‐catenin (β‐catenin FLAG ) and MYC‐tagged TCF‐4 (TCF‐4 MYC ). Cells were treated with 50 mM LiCl and 60 μM 15 for 24 h. β‐Catenin FLAG was immunoprecipitated from total protein extracts using anti‐FLAG‐conjugated agarose beads (left panel; IP: immunoprecipitation). Coimmunoprecipitated proteins were analyzed by Western blotting using an anti‐MYC‐tag antibody to detect TCF‐4 MYC (left panel; IB: immunoblot). Input shows the levels of TCF‐4 MYC and β‐catenin FLAG in 5% whole‐cell lysates before immunoprecipitation (right panel).
    Pcdna Myc Tcf 4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcdna myc tcf 4/product/Addgene inc
    Average 92 stars, based on 20 article reviews
    pcdna myc tcf 4 - by Bioz Stars, 2026-05
    92/100 stars

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    1) Product Images from "4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein"

    Article Title: 4‐(5‐Chloro‐3‐(3,4,5‐trimethoxybenzoyl)‐1 H ‐indol‐1‐yl)benzenesulfonamide: A Novel Polypharmacology Agent to Target Carbonic Anhydrase IX and XII With Improved Selectivity, Wnt/β‐Catenin Signaling Pathway, and P‐Glycoprotein

    Journal: Chemmedchem

    doi: 10.1002/cmdc.202500996

    Compound 15 inhibits the β‐catenin/TCF‐4 interaction. A coimmunoprecipitation assay was performed in HEK293T cells transiently transfected with FLAG‐tagged β‐catenin (β‐catenin FLAG ) and MYC‐tagged TCF‐4 (TCF‐4 MYC ). Cells were treated with 50 mM LiCl and 60 μM 15 for 24 h. β‐Catenin FLAG was immunoprecipitated from total protein extracts using anti‐FLAG‐conjugated agarose beads (left panel; IP: immunoprecipitation). Coimmunoprecipitated proteins were analyzed by Western blotting using an anti‐MYC‐tag antibody to detect TCF‐4 MYC (left panel; IB: immunoblot). Input shows the levels of TCF‐4 MYC and β‐catenin FLAG in 5% whole‐cell lysates before immunoprecipitation (right panel).
    Figure Legend Snippet: Compound 15 inhibits the β‐catenin/TCF‐4 interaction. A coimmunoprecipitation assay was performed in HEK293T cells transiently transfected with FLAG‐tagged β‐catenin (β‐catenin FLAG ) and MYC‐tagged TCF‐4 (TCF‐4 MYC ). Cells were treated with 50 mM LiCl and 60 μM 15 for 24 h. β‐Catenin FLAG was immunoprecipitated from total protein extracts using anti‐FLAG‐conjugated agarose beads (left panel; IP: immunoprecipitation). Coimmunoprecipitated proteins were analyzed by Western blotting using an anti‐MYC‐tag antibody to detect TCF‐4 MYC (left panel; IB: immunoblot). Input shows the levels of TCF‐4 MYC and β‐catenin FLAG in 5% whole‐cell lysates before immunoprecipitation (right panel).

    Techniques Used: Co-Immunoprecipitation Assay, Transfection, Immunoprecipitation, Western Blot



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    Image Search Results


    Compound 15 inhibits the β‐catenin/TCF‐4 interaction. A coimmunoprecipitation assay was performed in HEK293T cells transiently transfected with FLAG‐tagged β‐catenin (β‐catenin FLAG ) and MYC‐tagged TCF‐4 (TCF‐4 MYC ). Cells were treated with 50 mM LiCl and 60 μM 15 for 24 h. β‐Catenin FLAG was immunoprecipitated from total protein extracts using anti‐FLAG‐conjugated agarose beads (left panel; IP: immunoprecipitation). Coimmunoprecipitated proteins were analyzed by Western blotting using an anti‐MYC‐tag antibody to detect TCF‐4 MYC (left panel; IB: immunoblot). Input shows the levels of TCF‐4 MYC and β‐catenin FLAG in 5% whole‐cell lysates before immunoprecipitation (right panel).

    Journal: Chemmedchem

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    doi: 10.1002/cmdc.202500996

    Figure Lengend Snippet: Compound 15 inhibits the β‐catenin/TCF‐4 interaction. A coimmunoprecipitation assay was performed in HEK293T cells transiently transfected with FLAG‐tagged β‐catenin (β‐catenin FLAG ) and MYC‐tagged TCF‐4 (TCF‐4 MYC ). Cells were treated with 50 mM LiCl and 60 μM 15 for 24 h. β‐Catenin FLAG was immunoprecipitated from total protein extracts using anti‐FLAG‐conjugated agarose beads (left panel; IP: immunoprecipitation). Coimmunoprecipitated proteins were analyzed by Western blotting using an anti‐MYC‐tag antibody to detect TCF‐4 MYC (left panel; IB: immunoblot). Input shows the levels of TCF‐4 MYC and β‐catenin FLAG in 5% whole‐cell lysates before immunoprecipitation (right panel).

    Article Snippet: HEK293T cells were transfected with pcDNA/Myc TCF‐4 (Addgene #16512) and human β‐catenin pcDNA3 (#16828 Addgene).

    Techniques: Co-Immunoprecipitation Assay, Transfection, Immunoprecipitation, Western Blot